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Fig. 2 | Molecular Cancer

Fig. 2

From: Targeted deletion of CD244 on monocytes promotes differentiation into anti-tumorigenic macrophages and potentiates PD-L1 blockade in melanoma

Fig. 2

The deletion of CD244 on monocyte-lineage cells enhanced IFN-γ secretion from antigen-specific T cells. (A-E) The CD45+ cell population infiltrating the tumor was isolated using magnetic sorting and subjected to flow cytometry analysis 14 days after inoculating 1 × 106 B16F10 cells into CD244fl/fl and CD244fl/flLysMcre mice. IFN-γ and granzyme-B expression were measured after 16 h co-culture with γ-irradiated B16F10 cells. Expression of IFN-γ (A) and granzyme-B (B) in CD8 T cells, as well as IFN-γ expression in CD4 T cells (C), gp100-specific TCR (D), CD44 (E) expression in CD8 T cells and IFN-γ expression in CD44− and CD44+ CD8 T cells (left) and CD4 T cells (right) (F) were shown. *P < 0.05; ***P < 0.001; unpaired Student’s t-test. Data are representative of two (B, E, F) or compiled from two (A, C, D) independent experiments

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Molecular Cancer

ISSN: 1476-4598