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Fig. 2 | Molecular Cancer

Fig. 2

From: The construction of modular universal chimeric antigen receptor T (MU-CAR-T) cells by covalent linkage of allogeneic T cells and various antibody fragments

Fig. 2

CRISPR/Cas9-mediated TCR and HLA-I knockout did not affect the function of T cells and reduced alloreactivity. A The expression of CD5-CD30-CAR on non-disrupted and TCR−/HLA-I− CD8+ T cells. tCD19 was in-frame expressed with CD5-CD30-CAR to indicate the expression of CAR molecules. B The killing results of Karpas 299 target cells under different effector/target ratios. Data represented as mean ± SD. N = 3 independent biological replicates. C CD5-CD30-CAR-transduced CD8+ T cells derived from non-disrupted and TCR−/HLA-I− cells were co-cultured with Karpas 299 at a ratio of 4:1 respectively. IFN-γ secretion within each group was analyzed by ELISPOT assay. Data represented as mean ± SD. N = 3 independent biological replicates. D and E The inhibition of alloreactivity upon TCR/HLA-I disruption was determined utilizing the IFN-γ ELISPOT assay. Allogenic PBMCs were co-cultured with irradiated gene-edited T cells (D). Irradiated allogenic PBMCs were also co-cultured with gene-edited T cells (E). Data represented as mean ± SD. N = 7 independent biological replicates. Statistical analysis was performed by Student’s t-test. **** p < 0.0001

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Molecular Cancer

ISSN: 1476-4598