Fig. 3
LINC01852 promotes chemosensitivity in CRC cells. (A, B) A CCK-8 assay was used to investigate the effects of LINC01852 overexpression and knockdown on the sensitivity of CRC cells to 5-FU and L-OHP. CRC cells were exposed to gradually increasing concentrations of 5-FU and L-OHP for 48 h. (C) The LINC01852 expression level and the sensitivity to 5-FU and L-OHP were measured in HCT8-5R and HCT116-OR cells by qRT-PCR and CCK-8 assays, respectively. (D, E) The effects of LINC01852 overexpression on the colony formation (D) and apoptosis (E) of CRC cells treated with 5-FU (10 µM for HCT116; 40 µM for HCT8) or L-OHP (2 µM for HCT116; 8 µM for HCT8) for 14 days (colony formation, D) or 48 h (apoptosis, E). (F) The effects of LINC01852 overexpression on the in vivo tumorigenicity and chemosensitivity of CRC cells. Nude mice (n = 5) were injected with 2 × 106 LINC01852-overexpressing HCT116 cells. Seven days after tumor implantation, 5-FU (50 mg/kg) and L-OHP (10 mg/kg) were intraperitoneally injected into the mice every 3 days. *P < 0.05, **P < 0.01, ***P < 0.001