Fig. 8

Cotransfection analysis of circPDHK1 siRNA and HIF-2A overexpression vector in Caki-1 and 786-O cells. A Colony formation assays, (B) CCK-8 assays and (C, D) EdU assays were performed to detect the cell viability and proliferation activity of Caki-1 and 786-O cells transfected with circPDHK1 siRNA and/or HIF-2A overexpression vector. Bars = 200 μm (E–G) Transwell migration and invasion assays to detect cell migration ability of Caki-1 and 786-O cells cotransfected with the control siRNA and pCDNA3.1 vector, a circPDHK1 siRNA and pCDNA3.1 vector, the control siRNA and HIF-2A overexpressed vector, the circPDHK1 siRNA and the HIF-2A overexpressed vector. Bars = 100 μm. H Graphical depiction of circPDHK1 encoding PDHK1-241aa, which binds to PPP1CA to inhibit AKT dephosphorylation and activate the AKT-mTOR signaling pathway, thereby promoting the proliferation and metastasis of ccRCC. The schematic was drawn by Figdraw (https://www.figdraw.com). *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance