Fig. 1

General workflow. A: DNA was isolated of liquid and solid biopsies derived from different patient cohorts. Sequencing libraries were prepared that underwent methyl-binding domain (MBD) enrichment. The enriched fragments were sequenced by means of NGS to generate MBD-Seq data. B: CfMBD-Seq data together with already published regions from literature and public tissue data were used to identify differentially methylated regions (DMRs) that served for the design of a targeted panel. The panel was used for hybridization and capture with subsequent sequencing to enable high-throughput identification of different patient subgroups