Fig. 1

Identification and expression of circRPPH1 in TNBC. a Schematic diagram of circRPPH1 cyclization, and Sanger sequencing verified the backsplicing sequence and cyclization site of circRPPH1. b Relative expression levels of circRPPH1 in TNBC cell lines were analyzed by qRT-PCR. c Divergent primers detected circRPPH1 in cDNA but not in gDNA, and GAPDH was used as a negative control. d and e TNBC cells were analyzed for the abundance of RNase R-treated circRPPH1 and linear RPPH1 mRNA at the indicated time points. f Nucleo-plasmic separation experiments showed that circRPPH1 was predominantly localized in the cytoplasm of TNBC cells. GAPDH and U6 were used as internal references for cytoplasmic and nuclear transcripts, respectively. g FISH was performed to detect the localization of circRPPH1 in TNBC tissues (magnification, × 100, scale bar, 50 μm) and cells (magnification, × 200, scale bar, 20 μm). DAPI stained nuclei. h Mean fluorescence intensity analysis of TNBC tissue and normal breast tissue in FISH assay. i The relative expression levels of circRPPH1 in 60 pairs of TNBC tissues were analyzed by qRT-PCR. j The diagnostic value of circRPPH1 for TNBC was evaluated using ROC curves (cut-off value:0.367). k Overall survival analysis of 60 TNBC patients based on circRPPH1 expression