Fig. 2

ZNF460 increases circRPPH1 expression in TNBC cells. a and b Detection of ZNF460 expression in TNBC cells after transfection with ZNF460 overexpression plasmid or siRNA. c and d Detection of RPPH1 expression in TNBC cells after overexpression or knockdown of ZNF460 by qRT-PCR. e Plot of base frequency analysis of the binding site of ZNF460 to the RPPH1 promoter. f Schematic representation of the wild-type (WT) and mutant (Mut) sequences of the three predicted binding sites on the promoter of ZNF460 and RPPH1. g and h Relative luciferase activities were determined after co-transfection of TNBC cells with luciferase reporter plasmids containing wild-type or mutant RPPH1 promoter sequences and ZNF460 overexpression plasmids. i ChIP-qPCR assay to verify the binding site of the RPPH1 promoter to ZNF460 in TNBC cells, IgG was used as a negative control. j TNBC cells were analyzed for circRPPH1 expression after transfection with ZNF460 overexpression plasmid or siRNA