Fig. 5

circRPPH1 binds to miR-326 and acts as its molecular sponge. a Venn diagram showing the intersection of CircInteractome and ENCORI database circRPPH1 target miRNAs. b Relative expression of miR-330-5p and miR-326 in cell lines. c Relative expression of miR-326 in 60 pairs of TNBC tissues and adjacent normal tissues. d Overall survival curves were plotted based on miR-326 expression in 60 pairs of TNBC patients. e Pearson correlation analysis showed that miR-326 was negatively correlated with circRPPH1 expression in 60 pairs of TNBC tissues. f and g The relative expression of miR-326 was detected after overexpression or knockdown of circRPPH1 in TNBC cells. h Schematic diagrams of the binding sites of circRPPH1 and miR-326 and the circRPPH1-Wt and circRPPH1-Mut luciferase reporter vectors. i Relative activities of luciferase in 293 T cells were assayed after transfection of circRPPH1-Wt or circRPPH1-Mut and miR-326 mimics or mimics NC, respectively. J and k FISH experiments showed co-localization of circRPPH1 with miR-326 in tumor cells (magnification × 100, scale bar, 50 μm) and cells (magnification, × 200, scale bar, 20 μm). l and m RNA pull-down in TNBC cells with a biotin-labeled circRPPH1 probe followed by detection of circRPPH1 and miR-326 enrichment