Fig. 7

ITGA5 is a direct target of miR-326. circRPPH1 can act as a molecular sponge for miR-326 to regulate ITGA5 and activate the FAK/PI3K/Akt pathway. a and b The relative expression of ITGA5 was detected by qRT-PCR and western blot after transfection with miR-326 mimics and miR-326 inhibitor, respectively. c Schematic representation of luciferase reporter vectors containing wild-type (WT) and mutant (Mut) ITGA5 3'-UTR sequences. d Relative luciferase activity was determined after co-transfection of 293 T cells with ITGA5 3'-UTR WT/Mut luciferase reporter vectors and miR-326 mimics or negative controls, respectively. e Relative expression of ITGA5 was detected in 60 pairs of TNBC tissues and normal breast tissues. f Pearson correlation analysis showed that ITGA5 was negatively correlated with miR-326 and positively correlated with circRPPH1 in 60 pairs of TNBC tissues and normal breast tissues. g Relative expression of ITGA5 after overexpression or knockdown of circRPPH1 in TNBC cells. h and i Relative expression of ITGA5 after transfection or co-transfection of circRPPH1 overexpression vector, si-circRPPH1, miRNA mimics or inhibitors in TNBC cells. j and k Western blot detection of ITGA5 and FAK/PI3K/Akt pathway protein expression levels in TNBC cells after transfection or co-transfection with circRPPH1 overexpression vector, si-circRPPH1, miRNA mimics or inhibitors