Skip to main content
Fig. 4 | Molecular Cancer

Fig. 4

From: CircPCNXL2 promotes tumor growth and metastasis by interacting with STRAP to regulate ERK signaling in intrahepatic cholangiocarcinoma

Fig. 4

CircPCNXL2 modulates the activation of ERK/MAPK signaling pathway in ICC. (a) Volcano plot of the differentially expressed genes in circPCNXL2 overexpression group and control group. (b) KEGG pathway analysis of differentially expressed genes. (c) Western Bolt analysis showed the level of three subgroups of the MAPK signaling pathway in HuCCT1 and RBE cells. (d-e) Western blot analysis of the p-ERK and ERK in HuCCT1 and RBE cells. (f) EdU assay was conducted to evaluate the proliferation in HuCCT1 cells, scale bar = 50 μm. (g) CCK8 assay of HuCCT1 cells. (h) Colony formation assay of HuCCT1 cells. (i) Wound healing assay was used to evaluate the migration in HuCCT1 cells, scale bar = 100 μm. (j) Transwall assay was performed in HuCCT1 and RBE cells transfected with circPCNXL2 siRNAs or circPCNXL2 plasmid, Scale bar = 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Molecular Cancer

ISSN: 1476-4598