Fig. 3

Pharmacological FGFR inhibition elicits Janus-faced response patterns in non-irradiated and irradiated 3D lrECM HNSCC models. A Mean cell viability (± range) of cell models to indicated FGFRi concentrations (n = 3) at non-irradiated (left panel) or 6 Gy X-ray irradiated (right panel) conditions. Normalization was performed to corresponding non-irradiated/irradiated controls. B Plating efficiencies and surviving fractions of indicated cell models treated with FGFRi with or without a single dose of 6 Gy X-rays based on the colony number counts (n = 3; mean ± range; paired t-test; *p ≤ 0.05). C Normalized colony area of indicated cell models treated with FGFRi. The absolute area was normalized to the mean of the corresponding non-irradiated control (n = 3; mean ± range; paired t-test; **p ≤ 0.01, *p ≤ 0.05). D Representative focus stacked bright-field images of colony formation assays of 3D lrECM HNSCC cell models exposed to FGFRi (DMSO used as control) plus/minus 6 Gy X-ray irradiation. E Cell viability of 3D lrECM grown UM-SCC 10a cells upon single or double siRNA-mediated knockdowns of indicated genes under non-irradiated and 6 Gy X-ray irradiated conditions (n = 3; mean; two-way ANOVA; Tukey multiple comparison test; ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05). Non-targeting siRNAs were used as control