Fig. 1

FAP expression in the TME of NSCLC and its clinical significance. A The mRNA expression levels of FAP in tumor tissues and normal tissues in the LUAD and LUSC cohorts from the TCGA database. B Distinct fibroblast clusters in NSCLC _GSE127465 database as visualized by single-cell analysis. C The expression of FAP in different fibroblast clusters. D The cell development pseudotime constructed by CellTracer tools (http://bio-bigdata.hrbmu.edu.cn/CellTracer). The cells were ordered in a pseudo-temporal manner using Monocle2. E FAP expression in cellular developmental pseudotime in different branches. F The dynamic variation in functional activities along developmental pseudotime of branch 1. G Representative images of immunohistochemistry for high- or low-FAP expression in NSCLC tissues. (brown = FAP; blue = hematoxylin nuclear staining). Scale bar = 50 μm. H The proportion of patients with high/low stroma fraction in high/low FAP expression groups. I The proportion of patients with different metastasis status in high/low FAP expression groups. J Kaplan–Meier curves with patients stratified according to FAP expression (low vs. high) for OS. K Kaplan–Meier curves for LUAD and LUSC patients according to FAP expression levels in the TCGA database