Fig. 3

The functional implications of ARNTL2 and its relationship with EGFR in PC. A mRNA expression of ARNTL2 in PC from TCGA cohort and normal tissues from GTEx cohort; B Protein expression of ARNTL2 in PC and normal tissues; C IHC score of ARNTL2 in pancreatic cancer TMAs; D Kaplan–Meier survival analysis of the correlation between ARNTL2 expression and OS and DFS of PC patient in TCGA cohort; E The univariate Cox regression analysis were performed to depict the correlations between ARNTL2 expression and the clinicopathological features; F The protein expression levels of ARNTL2, EGFR and EGFR downstream signaling PI3K/AKT signaling pathway in PC cell lines (AsPC-1, BxPC-3, PANC-1, CFPAC-1, Capan-1, PATU-8988 T and MIAPaCa-2) and normal pancreatic duct cells (HPNE) were determined by western blot analysis; G The mRNA expression levels of ARNTL2 and EGFR in PC cell lines and normal pancreas cells were determined by qRT-PCR analysis. All data are presented as the mean ± SEM of triplicate experiments. *p < 0.05 by repeated measures with Student’s t-test