Fig. 1

Identifying and validating a novel oncogenic exosome circATP8A1 in Gastric Cancer. A Workflow diagram for isolating exosomes from the plasma of healthy individuals and gastric cancer patients. B TEM images of exosomes in the plasma of normal individuals and gastric cancer patients. C Nanoparticle tracking analysis (NTA) of exosomes in the plasma of normal individuals and gastric cancer patients. D Western Blot analysis of exosome markers CD81, TSG101. Calnexin as a negative control. E Heatmap of differential exosome circRNA expression in plasma between healthy individuals and gastric cancer patients. F Volcanic map of differential exosome circRNA expression in plasma between healthy individuals and gastric cancer patients. G The expression of circATP8A1 in gastric cancer tumor tissues (N = 100) and normal tissue (N = 42) was verified by qPCR. H Kaplan–Meier analysis of overall survival of circATP8A1 in our cohort. I & J The expression of circATP8A1 in gastric cancer tumor tissues and healthy individuals (Normal) was verified by digital PCR. Gastric cancer tumor tissues (N = 36), Normal (N = 36). Data are presented as mean ± SD. P value is determined by nonparametric test for G and J, log-rank text for H. *** P < 0.001