Fig. 4

High STAT6 expression in gastric cancer tissues and M2 polarized macrophages. A The TCGA database showed that STAT6 was highly expressed in gastric cancer tissues compared with adjacent tissues. B The expression level of STAT6 in normal and gastric cancer tissues was detected by immunohistochemistry. Scale bar = 100 μm. C The survival analysis of STAT6 was performed using the KM plot database. D Western blot analysis indicated that STAT6 was highly expressed in gastric cancer tissues compared with adjacent tissues. E Cells were grouped based on single-cell sequencing data. F The macrophages were grouped according to M1 and M2 markers. G Single-cell sequencing data showed that STAT6 expression was higher in M2 macrophages compared with M1 macrophages. H IF staining was performed with anti-STAT6 and anti-CD206 in gastric cancer tissue. Scale bar = 100 μm. I After THP1 cells were treated with PMA, the cells were treated with IL4 or AS1517499, and the related proteins were finally detected by western blot. *** P < 0.001. N(1–6), normal tissue; T(1–6), gastric cancer tissue