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Fig. 3 | Molecular Cancer

Fig. 3

From: Bladder-cancer-derived exosomal circRNA_0013936 promotes suppressive immunity by up-regulating fatty acid transporter protein 2 and down-regulating receptor-interacting protein kinase 3 in PMN-MDSCs

Fig. 3

Identifcation and characterization of circRNA_0013936. (A) The heatmap showed the differentially expressed circRNAs (> 4-fold) in BCa-exosomes and SV-HUC-1-exosomes. (B) Schematic diagram of the composition of circ_0013936. (C) qRT-PCR was used to detect the expression of circ_0013936 in exosomes derived from three bladder cancer cell lines (T24, UMUC-3 and BIU-87) and normal urothelial cell (SV-HUC-1). (D) Sanger sequencing and agarose gel electrophoresis were used to verify the back splicing sequence and RT-PCR product of circ_0013936. (E) Detection of circRNA_0013936 expression by real-time PCR in T24 and UMUC3 cells treated with or without RNase R. (F) FISH confirmed circRNA_ 0013936 is mainly located in the cytoplasm. (*P < 0.05)

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Molecular Cancer

ISSN: 1476-4598