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Fig. 5 | Molecular Cancer

Fig. 5

From: Bladder-cancer-derived exosomal circRNA_0013936 promotes suppressive immunity by up-regulating fatty acid transporter protein 2 and down-regulating receptor-interacting protein kinase 3 in PMN-MDSCs

Fig. 5

CircRNA_ 0013936 functions as a sponge for miR-320a and miR-301b-3p. (A) Evaluation of the effects of 9 candidate miRNAs on the luciferase activity of circ_0013936 using luciferase reporter gene assay. (B and C) The RNA pull-down assay was performed in PMN-MDSCs using circ_0013936 and negative probe. (D) The basic expressions of miR-320a and miR-301b-3p in PMN-MDSCs vs. PMNs. (E) Schematic diagram of mutant (Mut) or wild-type (Wt) circ_0013936 luciferase reporter vectors carrying miR-320a or miR-301b-3p binding sites. (F and G) The relative expressions of miR-320a and miR-301b-3p detected by qRT-PCR in 293T cells transfected with mutant (Mut) or wild-type (Wt) circ_0013936. (* P < 0.05)

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Molecular Cancer

ISSN: 1476-4598