Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: Bladder-cancer-derived exosomal circRNA_0013936 promotes suppressive immunity by up-regulating fatty acid transporter protein 2 and down-regulating receptor-interacting protein kinase 3 in PMN-MDSCs

Fig. 6

JAK2 is the direct target gene for miR-320a, and CREB1 is the direct target gene for miR-301b-3p. (A and G) Venn diagram showing the predicted target genes of miR-320a and 301b-3p. (B and H) qRT-PCR detected changes in mRNA levels of predicted targets overexpressed or silenced by circ_0013936. (C and D) Relative luciferase protease activity measured by luciferase reporter gene assay in 293T cells transfected with JAK2 (Mut or Wt) and miR-320a mimics. (E and F) Detection of JAK2 expression in PMN-MDSCs transfected with miR-320a inhibitors or miR-320a mimics using Western blotting and qRT-PCR. (J and K) Relative luciferase activity measured by luciferase reporter gene assay in 293T cells transfected with CREB1 (Mut or Wt) and miR-301b-3p mimics. (L and M) Detection of CREB1 expression in PMN-MDSCs transfected with miR-301p-3p inhibitors or miR-301b-3p mimics using Western blotting and qRT-PCR (*P < 0.05)

Back to article page

Molecular Cancer

ISSN: 1476-4598