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Fig. 7 | Molecular Cancer

Fig. 7

From: Bladder-cancer-derived exosomal circRNA_0013936 promotes suppressive immunity by up-regulating fatty acid transporter protein 2 and down-regulating receptor-interacting protein kinase 3 in PMN-MDSCs

Fig. 7

CircRNA_0013936 up-regulated FATP2 and down-regulated RIPK3 through the miR-320a/JAK2 and miR-301b-3p/CREB1 pathway in PMN-MDSCs. (A, B and C) The expressions of JAK2 and FATP2 in PMN-MDSCs after over-expressing or silencing circ_0013936 were detected by qRT-PCR and Western blotting. (D, E and F) The FATP2 expression in PMN-MDSCs after over-expressing or silencing of JAK2 were detected by qRT-PCR and Western blotting. (G) Detection of TG, AA, and PGE2 in PMN-MDSCs transfected with vector, lv-circ_0013936, mimic-miR-320a or mimic-nc using LC-MS. (H, I and J) Detection of CREB1 and RIPK3 expression in PMN-MDSCs after over-expressing or silencing circ_0013936 using Western blotting and qRT-PCR. (K, L and M) Detection of RIPK3 expression in PMN-MDSCs after silencing or over-expressing CREB1 using qRT-PCR and Western blotting. (N) Detection of immunosuppressive molecules (IL-10, INOS, Arg-1) in PMN-MDSCs transfected with vector, lv-circ_0013936, mimic-miR-301b-3p or mimic-nc using ELISA. (*P < 0.05)

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Molecular Cancer

ISSN: 1476-4598