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Fig. 8 | Molecular Cancer

Fig. 8

From: Bladder-cancer-derived exosomal circRNA_0013936 promotes suppressive immunity by up-regulating fatty acid transporter protein 2 and down-regulating receptor-interacting protein kinase 3 in PMN-MDSCs

Fig. 8

Exosomal circRNA_0013936 promotes suppressive immunity by up-regulating FATP2 through miR-320a/JAK2 pathway and down-regulating RIPK3 through miR-301b-3p/CREB1 pathway in PMN-MDSCs. (A and B) Detection of mRNA expression of JAK2, pSTAT5, and FATP2 in PMN-MDSCs co cultured with BCa derived exosomes or circRNA_0013936-KO-BCa derived exosomes using qRT-PCR and Western blotting. (C) Detection of TG, AA, and PGE2 in PMN-MDSCs using LC-MS. (D and E) Detection of mRNA expression of CREB1 and RIPK3 in PMN-MDSCs using qRT-PCR and Western blot. (F) Detection of immunosuppressive molecules (IL-10, INOS, Arg-1) in PMN-MDSCs using ELISA. (G) Flow cytometry was performed to evaluate the effect of PMN-MDSCs cocultured with BCa-derived exosomes or circRNA_0013936-KO-BCa-derived exosomes on the IFN-γ production of CD8+ T cells. (H) CFSE analysis was performed to evaluate the effect of PMN-MDSCs cocultured with BCa-derived exosomes or circRNA_0013936-KO-BCa-derived exosomes on the proliferation function of CD8+ T cells. (*P < 0.05)

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Molecular Cancer

ISSN: 1476-4598