Fig. 3

Biopsies immune profiles and lymphocyte count. A-B (Top panels). Absolute lymphocyte blood counts from baseline evaluation to the fifth cycle of atezolizumab (D84) in patients who experienced stable disease, partial response or complete response as best tumor response according to RECIST 1.1 (SD/PR/CR) (A) and in patients who rapidly progressed (PD) (B). Red arrows indicate SABR delivery during the course of treatment. The three periods of tumor biopsies are also indicated. Yellow and red stars refer respectively to MSI-high patients and elite responders (PFS > 1 year). (Bottom panels). Immunograms detailing the immune composition of the immune infiltrate as assessed by multiplex IHC in FFPE tumor biopsy samples collected at the indicated timepoints in patients with SD/PR/CR (A) an in patients who progressed (B). Each absolute cell density is ranged from 0 to 10.000 cells/mm2. C-F Multiplex IHC. Images at 20X of two liver samples with a high level (C) and a low level (D) of immune infiltration; (E); Comparison of tumor immune infiltration according to disease control rate (defined as lack of disease progression) in baseline biopsies by different IHC markers; (F) Scatter dot plot showing the sum of densities of immune infiltrating cells assessed by multiplex IHC in tumor samples and calcutated as follows: (macrophages density + CD3 + cells density + CD68 + density + CD20 + density). Mean with SD are represented. ns > 0.05; 0.05 ≤ * < 0.01; 0.01 ≤ ** < 0.001; 0.001 ≤ *** < 0.0001; 0.0001 ≤ ****. HES: Haematoxylin–Eosin-Saffran; d: DAB brown; p: purple; y: yellow; g: green