Fig. 1

LINC00115 is significantly upregulated in paclitaxel-resistant breast cancer stem-like cells. A A heatmap of lncRNAs differentially expressed in paclitaxel-resistant (PTX_R) MDA-MB-231 BCSCs and non-BCSCs. The top 100 lncRNAs with significant differences are shown in the heatmap. B QRT-PCR of LINC00115 expression in non-BCSCs and BCSCs from various PTX-resistant breast cancer cells. C High LINC00115 expression correlates with reduced overall survival (OS) in breast cancer specimens after endocrine and chemotherapy in the Kaplan-Meier plotter dataset. The cutoff value used in the analysis is -3.32. D QRT-PCR of LINC00115 knockdown (KD) using two different LINC00115 shRNAs (sh115-1 and sh115-2) or a control shRNA (shC) in PTX-resistant BCSCs and non-BCSCs.E. Representative images of LINC00115 KD on mammospheres formation of LINC00115-knockdown BCSCs derived from MDA-MB-231/PTX_R and BT549/PTX_R cells. Scale bars, 12.5 μm. F Quantification of mammospheres formation in (E). G Representative bioluminescence images of LINC00115 KD-decreased tumor metastasis (n = 6). Mice were imaged at 14 days after tail vein injection. H Quantification of the bioluminescence activity in (G). I Representative hematoxylin and eosin (H & E) staining (upper panel) and bright-field imaging (lower panel) of the lungs in (G). Scale bars: 100 μm. J The number of macroscopic lesions on the lung surfaces in (G) was quantified at necropsy. K Kaplan-Meier survival of animals with indicated MDA-MB-231 BCSC tumors (n = 6).Data are representative of three independent experiments with similar results. Error bars, ± SEM. **P < 0.01, ***P < 0.001, by paired two-tailed t-test or log-rank test