Fig. 2

TRPM2-AS promotes GBC angiogenesis in vivo. A, B Representative images and quantification of surgically removed GBC tumors at day 28 in the TRPM2-AS overexpression group (A) and at day 20 in the TRPM2-AS knockdown group (B) (n = 6). C, D Microvascular density of GBC tumor sections in the TRPM2-AS overexpression (C) and knockdown (D) groups was evaluated by CD34 immunohistochemical staining. Scale bar: 10/50 μm. E, F Representative images of bioluminescence imaging at day 40 showing the pulmonary metastasis in the TRPM2-AS overexpression (E) and knockdown (F) groups (n = 5). G, H Representative images of surgically removed lung tissues and H&E-staining showing the pulmonary metastasis in the TRPM2-AS overexpression (G) and knockdown (H) groups. Scale bar: 300 μm. I, J Representative immunohistochemical staining images and quantification of dissected lung tissues detecting the microvascular density in the TRPM2-AS overexpression (I) and knockdown (J) groups. Scale bar: 50/200 μm. Data were assessed with unpaired Student’s t test, one-way ANOVA, or two-way ANOVA and presented as mean ± SD. ** P < 0.01; *** P < 0.001