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Fig. 8 | Molecular Cancer

Fig. 8

From: Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation

Fig. 8

SERPINE1 promoted NF-κB p65 nuclear translocation. (A) GFP-SERPINE1 plasmids were transfected into 293T cells, the GFP-Mock plasmids were used as control. We immunoprecipitated extracts from whole cell lysates with anti-GFP antibody and analyzed the resulting complexes by LC-MS/MS. Venn diagram showed the proteins pulled down in GFP-SERPINE1 group and GFP-Mock group. (B and C) Co-IP of endogenous interaction of p65 and SERPINE1 in RKO cells. (D) Co-IP of exogenous interaction of p65 and SERPINE1 in 293T cells. (E and F) CRC cells were co-cultured with Sen-EVs (15 µg/ml) for 48 h. (E) Cellular distribution of SERPINE1, p65, and p-p65 was detected. (F) Representative images of immunofluorescence staining displaying the co-localization of SERPINE1 (red) and p65 (green). DAPI: blue. Line chart of fluorescence signal positioning analysis

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Molecular Cancer

ISSN: 1476-4598