Figure 2

Gene expression analysis. C4-2B (solid lines) and LNCaP cells (broken lines) were maintained in 5% CSS-containing medium for three days, and then re-fed (time 0) with the same medium supplemented with either 10 nM DHT or ethanol vehicle. RNA was extracted at the indicated time points during the time course and expression of the specified genes was measured by RT-qPCR. Expression levels relative to 18S rRNA (which itself stayed stable throughout the time course) are shown with the 0 time values defined as 1 for each cell line. Representative data is shown from one of two independent experiments with n = 3, except for panels 2L, O, U, Y and ZC, where the C4-2B data is derived from 6 measurements (see Additional file 3 for the complete set of raw data). Error bars are SEM. Genes are roughly ordered based on the DHT-responsiveness in C4-2B cells, with stimulated genes first (panels A-J) to repressed genes last (panels ZC-ZF). TRVP3 mRNA was barely detectable in LNCaP cells.