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Figure 8 | Molecular Cancer

Figure 8

From: Selective repression of retinoic acid target genes by RIP140 during induced tumor cell differentiation of pluripotent human embryonal carcinoma cells

Figure 8

RIP140 silencing sensitizes NT2/D1 cells to low dose RA and accelerates and amplifies RA-dependent expression of RA-target genes. (A) Immunophenotypic FACS analysis for the established neuroectodermal marker A2B5 in NT2/D1 cells transfected with 40 nM scrambled siRNA (Scbl) or RIP140 siRNA#1 or RIP140 siRNA#2 and treated for 4 days with DMSO vehicle control or indicted dosages of RA. Data are presented as mean fluorescence (left) and percent positive cells (right). (B) RT-PCR analysis of NT2/D1 cells transfected with 60 nM scrambled siRNA (Scbl) or RIP140 siRNA#1 and treated with 10 μM RA for the indicated time points. Representative of two experiments. (C) Models of RIP140 regulation of RA signaling supported by the current study. See text for details.

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Molecular Cancer

ISSN: 1476-4598