The SAR domain contains the region that binds α-ESE-1 mAB405 antibody. Immunofluorescence and direct fluorescence images of MCF-12A cells transiently transfected with GFP-SAR (SAR) and GFP-SAR-myc Box 1 - Box 4 mutant constructs (labeled SAR-myc Box 1 - SAR-myc Box 4, respectively). All cells were stained with anti-ESE-1 antibody mAB405 followed by secondary anti-mouse IgG conjugated to Cy3. DAPI - blue channel, DAPI fluorescence. Cy3 (αESE-1) - red channel, indirect immunofluorescence signal indicating binding of mAB405. GFP - green channel, direct fluorescence of GFP fusion protein. Merge - overlay of blue, red and green channels.