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Figure 6 | Molecular Cancer

Figure 6

From: A fiber-modified adenoviral vector interacts with immunoevasion molecules of the B7 family at the surface of murine leukemia cells derived from dormant tumors

Figure 6

B7-mediated gain of Ad5FB4-permissiveness by HeLa cells. HeLa cells were transfected with B7.1- and B7-H1-expressing plasmids prior to transduction by Ad5FB4 at 500 vp/cell. Cells were fixed and stained with X-gal substrate at 48 h after incubation with Ad5FB4, and ß-gal activity (expressed as arbitrary units; AU) was determined by light microscopy, using the Image J software (n = 4 per condition). (a), Control, Ad5FB4-transduced, nontransfected cells. (b), Ad5FB4-transduced cells expressing B7.1 alone. (c), Ad5FB4-transduced cells expressing B7-H1 alone. (d), Ad5FB4-transduced cells coexpressing B7.1 and B7-H1. (e), Ad5FB4-transduced cells coexpressing B7.1-Rluc8 and B7-H1-YPet fusion proteins. (f), Ad5FB4-transduced cells coexpressing B7-H1-Rluc8 and B7.1-YPet. Symbols: **, p < 0.01; NS, non significant.

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Molecular Cancer

ISSN: 1476-4598