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Figure 6 | Molecular Cancer

Figure 6

From: Mesothelin confers pancreatic cancer cell resistance to TNF-α-induced apoptosis through Akt/PI3K/NF-κB activation and IL-6/Mcl-1 overexpression

Figure 6

Inhibiting NF-κB/Akt pathway or endogenous IL-6 abrogates MIA-MSLN cells resistance to TNF-α-induced apoptosis. (A). MIA-MSLN cell viability was reduced upon pre-treatment with IKK inhibitor wedelolactone and then 20 ng/ml of TNF-α for 72 h. (B). MIA-MSLN cell viability was reduced upon pre-treatment with PI3K inhibitor Ly 2940002 and then 20 ng/ml of TNF-α for 72 h. (C). Cell apoptosis was determined by TUNEL assay in samples treated as in (A) and (B). (D). Proteins from similarly treated cells were subjected to western blot to detect caspase 3 cleavage and key pro/anti-apoptotic molecules.

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