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Table 1 Percent of cells undergoing apoptosis by TNF-α treatment

From: Mesothelin confers pancreatic cancer cell resistance to TNF-α-induced apoptosis through Akt/PI3K/NF-κB activation and IL-6/Mcl-1 overexpression

Cell Line

% TUNEL positive

(without TNF-α)

% TUNEL positive

(20 ng/ml TNF-α)

MIA

0.0

27.3

Capan-1

4.7

5.3

BxPC3

4.9

7.6

PL 45

4.5

2.9

AsPC-1

4.7

1.8

Capan-2

4.8

8.7

Panc 48

4.3

8.5

  1. Cells were plated in 6 well plates, starved over night and treated with 20 ng/ml of TNF-α for 72 h. Cells were collected, fixed, washed and then processed for TUNEL assay.
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Molecular Cancer

ISSN: 1476-4598