Figure 5

Plumbagin suppresses migration of breast and gastric cancer cells. A, The wound-healing assay for evaluating the inhibitory effect of plumbagin on MDA-MB-231 cell migration. Confluent monolayers of MDA-MB-231 cells were scarred, and repair was monitored microscopically after 6 h of pre-treatment with 5 μM plumbagin before being exposed to 100 ng/mL CXCL12 for 24 h. Width of wound was measured at time zero and 24 h of incubation with and without plumbagin in the absence or presence of CXCL12 in RPMI medium containing 1% serum. The representative photographs showed the same area at time zero and after 48 h of incubation. Graphs, mean (n = 3); bars, SE. *, P < 0.05. B, The wound-healing assay for evaluating the inhibitory effect of plumbagin on AGS cell migration. Confluent monolayers of AGS cells were scarred, and repair was monitored microscopically after 6 h of pre-treatment with 5 μM plumbagin before being exposed to 100 ng/mL CXCL12 for 24 h. Width of wound was measured at time zero and 24 h of incubation with and without plumbagin in the absence or presence of CXCL12 in RPMI medium containing 1% serum. The representative photographs of three independent experiments showed the same area at time zero and after 48 h of incubation. Graphs, mean (n = 3); bars, SE. *, P < 0.05.