Differential expression of miR-125b in pediatric AML patients. (A) Differential expression of miR-125b in normal samples and different subtypes of pediatric AML determined by miRNA microarray analysis (the same part of different microarray chips is shown). Bright green dots indicate highly expressed miRNAs. (B) Expression of miR-125b in the normal pool (N); different subtype pools of pediatric AML and M3 complete remission pool (M3 CR) were validated by northern blot. (C) Expression levels of miR-125b in subtypes of 131 pediatric AML were analyzed with qRT-PCR. Data are presented as the fold change of miR-125b expression in patient samples with respect to expression in bone marrow mononuclear cells (MNC) from 13 healthy donors. The average miR-125b expression of each subtype was statistically compared with the average normal value. *p < 0.05; **p < 0.01.