Figure 3
Increased Treg cells in the transgenic tissue. Cell suspensions from transgenic St3/4 and control (NSC) ear tissue (approximately 5 × 105 cells/sample) were analysed by flow cytometry using fluorescent-conjugated antibodies. The leukocyte populations were first gated by forward and side scatter as shown in figure 2. Cells were stained using antibodies directed to CD4(FITC), CD25(PE) and FoxP3(APC). CD4+CD25+ cells gated in the upper panel are shown for FoxP3 staining in the middle and lower panels. The proportions of cells in relevant quadrants or gates are given.