Myc associates with the Bim promoter by a RAD001 dependent process in BT474 cells. (A) Schematic representation of the potential Myc binding sites of the BCL2L11 promoter. (B) BT474 cells were treated (2, 4, 6) or not (1, 3, 5) with RAD001, soluble chromatin was immunoprecipitated with anti-Myc (3, 4) or anti-E2F-1 antibodies (5, 6) and DNA samples were then amplified using primers that cover the -61/+50 region of the BCL2L11 promoter. IgG immunoprecipitations were used as controls (1, 2). Data are mean ± sd of 4 independent experiments normalized to data obtained with control IgG immunoprecipitations. p-values were assessed using a Student's t test.