Figure 4From: Oncogenic functions of hMDMX in in vitro transformation of primary human fibroblasts and embryonic retinoblastshMDMX overexpression inhibits Nutlin-3 mediated p53 activation in human fibroblasts. A. Various VH10hTERT cell lines were continuously treated with 10 μM Nutlin-3 and proliferation was measured after 96 hours using a WST-1 assay. Relative cell numbers are displayed as survival relative to untreated cells. B. Various VH10hTERT cell lines were treated for the indicated times with 10 μM Nutlin-3, and protein levels were analyzed with immunoblotting using the indicated antibodies. C. qRT-PCR analysis of cells treated as in B. Expression levels of hMDM2-p2, p21, PUMA and SURVIVIN are shown as the fold induction relative to untreated wild-type VH10 cells.Back to article page