Figure 4

BRAFV600E enhances the migrating and invasive capacity of colon adenocarcinoma cells through RhoA activation. (A) GST-pull down assay for RhoA activation and quantification (upper and middle panel) and western blot analysis of pSer445BRAF levels in a panel of cell lines (lower panel). (B) Western blot analysis p-Cofilin that is enhanced in Caco-BR13 and Caco-BR23 cells as compared to the parental Caco-2 cells (upper panel) and quantification (lower panel).