Inhibition of RhoA-Rho kinase pathway in Caco-2 and Caco-BR cells. (A) Caco-2 and Caco-BR13 cells were treated with the Rho kinase inhibitor Y-27632 for 4 hours at indicated concentrations to achieve downregulation of p-Cofilin. (B) The effect of Y-27632 on cell migration in Caco-2 and Caco-BR cells. ***: P ≤ 0,001, as determined with Student t-test. (C) Cells were treated for 48 hours with 64 pmol of a negative siRNA control sequence (siCtr) or with 32 pmol siRNA against ROCK1 combined with 32 pmol against ROCK2. (D) Cell migration and invasion was determined following treatment with siRNA against ROCK1/2.