Cellular sensecence in response to Docetaxel treatment. The PC-3 Ag, PC-3 D8 and PC-3 D12 cells were plated in 6 well plates at 300,000/well and grown for 24 hours. The cells were then treated with Docetaxel (100 nM) for 48 hours and fixed with 2% formaldehyde and 0.2% glutaraldehyde. Senescent cells were then assessed by staining cells for β-galactosidase expression. Positive cells were counted and expressed as a percentage of the total number of cells in the well. Pictures are a representation of 5 independent experiments.