Characterisation and manipulation of docetaxel resistant prostate cancer cell lines

Table 1 Genes increased in the PC-3 D8 Docetaxel Resistant Cell line

PC-3 D8 vs PC-3 Ag Gene Name	Accession no.	Fold change	p value
BIK	NM_001197	4.606724005	0.024378
FOXO1	NM_002015	4.092077589	0.006372
NAIP	NM_004536	3.895819042	NA
HSP90AA1	NM_001017963	2.255183574	0.116769
IL8	NM_000584	2.194814925	0.060076
BCL2A1	NM_001114735	2.003718027	0.018472
NBN	NM_002485	1.994366021	0.011912
TNFRSF10C	NM_003841	1.931533233	0.288353
TP73	NM_001126240	1.785086313	0.082871
BNIP3L	NM_004331	1.747205486	0.03454
CLU	NM_001171138	1.740002897	0.304795
APAF1	NM_001160	1.673345235	0.38724
FASLG	NM_000639	1.563978305	0.73945
BNIP3	NM_004052	1.50139036	0.173556

Pre-designed TaqMan probe and primer sets for target genes were chosen from an on-line catalogue (Applied Biosystems). Expression levels of target genes were normalised to concentration of 18S rRNA (Additional File 1 lists the 95 genes chosen for inclusion and their function). Total mRNA was extracted from the PC-3 Ag and PC-3 D8 cells and used on custom designed Low Density Arrays (LDA) to determine which genes were increased (fold increase > 1.5) in the Docetaxel resistanct PC-3 D8 subline compared to the PC-3 Ag. n = 3.

ISSN: 1476-4598