Characterisation and manipulation of docetaxel resistant prostate cancer cell lines

Table 3 Genes increased in the PC-3 D12 Docetaxel Resistant Cell line

PC-3 D12 vs PC-3 Ag Gene Name	Accession no.	Fold change	p value
NAIP	NM_004536	28.14023	NA
BCL2A1	NM_001114735	9.956213	7.84E-06
CLU	NM_001171138	6.138126	0.019757
TNFRSF1B	NM_001066	4.568718	NA
ATM	NM_000051	2.884063	0.013665
FOXO1	NM_002015	2.746056	0.0167
MAP2K4	NM_003010	2.456245	0.13486
APAF1	NM_001160	2.264102	0.34641
NOL3	NM_001185057	1.946074	0.101148
CHEK1	NM_001114121	1.554884	0.433367
BNIP3L	NM_004331	1.514918	0.113779
NBN	NM_002485	1.505995	0.051555

Total mRNA was extracted from the PC-3 Ag and PC-3 D12 cells and used on custom designed Low Density Arrays (LDA) which included the transcriptional profile probes for the IAPs, death receptors, death ligands, and signalling molecules as well as genes involved in cell cycle regulation, DNA damage and repair and chemotherapy resistance. Additional File 1, lists the 95 genes chosen for inclusion, and their function. Genes whose expression has increased (fold increase > 1.5) in the Docetaxel resistanct PC-3 D12 subline compared to the PC-3 Ag. n = 3.

ISSN: 1476-4598