Immunocytochemical analysis of total STAT1 expression and subcellular localisation. DSL-6A/C1 cells treated with IFNγ (100 ng/ml) as indicated were stained by incubation with specific antibodies detecting STAT1. Upper row: Binding of primary antibody was visualized with an Alexa Fluor® 488-labeled secondary antibody, resulting in green fluorescence. Middle row: Nuclei were counterstained with TO-PRO®-3 iodide (shown in red color). Lower row: merged pictures. Microphotographs were analyzed by confocal laser scanning microscopy (original magnification × 400).