Figure 4
Spindle pole fragmentation in Aurora-Ai mitoses depends on Eg5 activity. A schematisation of the protocol is shown (time intervals not represented to scale). IF panels show spindles displaying normal or fragmented poles in control and Aurora-Ai cells, respectively (first and second row); monopolar spindles in Aurora-Ai cells treated with MON (third row); spindles displaying pole fragmentation in Aurora-Ai cells after MON release (MON-rel; lower row). Histograms represent the percentage of PM/M displaying fragmented poles, as assessed by alpha-tubulin (left) and pericentrin (right) staining (200 to 400 counted cells per condition in 2-4 experiments; s.d are shown). *: p < 0.01, **: p < 0.001, χ2 test. Scale bar: 10 μm.