Figure 2

Adv-Stat3(-) replicates adequately in MSCs and is eventually released. (A) Cytopathic effects of Adv-GFP, Adv-Stat3(-), and Adv-wt on MSCs. MSCs were infected with Adv-GFP, Adv-Stat3(-), and Adv-wt at different MOI. The cytotoxic activity was evaluated by crystal violet staining at each time point. The control without any viral infection is indicated as Ctr. (B) Viral count in the culture medium of MSCs was expressed as mean ± SD in three independent experiments. MSCs were infected with 500 MOI Adv-GFP, Adv-Stat3(-), and Adv-wt. At each time point, the culture medium was collected and the viral concentration measured using the TCID₅₀ method. (C) Intracellular viral concentration was expressed as mean ± SD in three independent experiments. MSCs were infected with 500 MOI Adv-GFP, Adv-Stat3(-), and Adv-wt. At each time point, the intracellular virus was extracted by three cycles of freezing and thawing and measured using the TCID₅₀ method. (D) DNA replication of Adv-GFP, Adv-Stat3(-), and Adv-wt in MSCs. MSCs were infected with 500 MOI Adv-GFP, Adv-Stat3(-), and Adv-wt. At each time point, MSCs were harvested and real-time PCR performed to measure the amount of viral DNA. Results are the means of three independent experiments and expressed as the ratio with GAPDH. Negative controls omitted templates.