Efficient transduction and stable knockdown of SOX2 in glioblastoma cell lines U343-MG and U373-MG. a: Western blot analysis showing knockdown of SOX2 in transduced U343-MG and U373-MG cells using pRVH-N1-EGFP vectors encoding the small hairpins sh788 and sh2378. As control, total protein lysates of cells transduced with shLuc are included. The membrane was stripped and probed for tubulin in order to confirm equal protein loading. b: Indirect immunofluorescence analysis of SOX2 expression in U343-MG and U373-MG cells after transduction of retroviral vectors encoding shRNA-shSOX2-788 and shRNA-SOX2-2378. Note the loss of SOX2-immunosignals in transduced EGFP-positive cells. Controls transduced with shLuc are included.