Figure 6
From: Anti-tumor effects of fibroblast growth factor-binding protein (FGF-BP) knockdown in colon carcinoma
Analysis of cellular and molecular effects of FGF-BP knockdown. The effects of FGF-BP levels on cell proliferation are dependent on p21 as indicated by the absence of anti-proliferative effects of FGF-BP knockdown in HCT-116 p21 -/- cells (A). Dependence of antiproliferative GSK3β effects on FGF-BP. GSK3β inhibition by treatment of LS174T cells with 6-bromoindirubin-3-oxime (BIO) leads to the induction of proliferation (B, left). This effect is abrogated upon FGF-BP knockdown (B, right). Dependence of FGF2 cell proliferation-simulating effects on FGF-BP expression. Stimulation of LS174T cells by treatment with FGF2 leads to the induction of proliferation (C, left). This effect is largely abrogated upon FGF-BP knockdown (C, right). Anti-proliferative effects of transient siFGF-BP transfection (D).