Figure 7

Anti-tumor effects of therapeutic FGF-BP knockdown in vivo. Upon establishment of s.c. LS174T tumor xenografts, tumor-bearing mice were randomized and treated at the indicated time points by systemic injection of 10 μg PEI-complexed siRNAs. PEI/siRNA-mediated knockdown leads to tumor growth inhibition reaching statistical significance between PEI/siFGF-BP and PEI/siNeg.ctrl. (A, left). Termination of the experiment upon occurrence of mechanical wounds in the tumors, resulting in the loss of tumor mass in some mice of the control-treated and untreated groups (A, right for representative examples of mice). Oberserved anti-tumor effects are based on the PEI-mediated delivery of intact siRNAs as determined by [32P]-labeled siRNA molecules (B), leading to reduced FGF-BP protein levels in the treatment group as determined by Western blotting (C; see right panel for representative examples).