Figure 3
From: PIAS1 interacts with FLASH and enhances its co-activation of c-Myb
Regulation of c-Myb activity by PIAS1 and FLASH. (A) CV-1 cells were transfected with the c-Myb-responsive 3×MRE(GG)-MYC reporter plasmid (0.2 μg) and plasmids encoding c-Myb, FLASH and PIAS1 as indicated (each 0.2 μg). (B) HD11 cells were transfected with plasmids encoding c-Myb, FLASH and PIAS1 as indicated. c-Myb activation of the target gene mim-1 was measured by quantitative real-time PCR with primers specific for mim-1 and HPRT. The results are presented as mim-1 expression relative to HPRT expressions ± SEM. (C) CV-1 cells were transfected with the c-Myb-responsive 3×MRE(GG)-MYC reporter plasmid (0.2 μg) and plasmids encoding c-Myb-2KR, FLASH and PIAS1 as indicated (0.2 μg each). The results in (A) and (C) are presented as relative luciferase units (RLU) and represent the mean RLU ± SEM of three independent assays performed in triplicates. For each experiment Western immunoblotting is shown below.