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Table 4 mRNA expressions of the 20 markedly upregulated genes in breast cancer cell lines (ERa-negative and ERa-positive) and in primary cell cultures of epithelial cells and fibroblasts from normal breast tissues and breast tumor cells

From: Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy

Genes

Normal breast tissues

(n = 9)

Breast tumor cell lines

(n = 12)

ERα-negative cell lines

(n = 7)

ERα-positive cell lines

(n = 5)

pa

Normal fibroblasts

Normal epithelial cells

Tumoral fibroblasts

Tumoral epithelial cells

AURKB

1,0 (0,00-3,24)

87,55 (20,39-163,71) b

118,19 (73,26-163,71)

32,90 (20,39-71,01)

< 0,01

1,11

3,39

5,41

22,55

TPX2

1,0 (0,31-4,23)

66,67 (23,37-123,35)

94,35 (59,99-123,35)

33,98 (23,37-47,50)

< 0,01

1,88

2,33

6,76

18,38

CDC20

1,0 (0,06-1,28)

25,90 (6,68-88,24)

38,68 (19,88-88,24)

10,22 (6,68-13,24)

< 0,01

0,77

0,48

2,42

4,99

BUB1

1,0 (0,00-1,38)

58,09 (16,34-155,96)

79,89 (39,81-155,96)

25,81 (16,34-45,41)

< 0,05

2,76

1,64

6,66

28,54

CCNA2

1,0 (0,05-1,45)

41,04 (9,75-79,34)

50,91 (18,64-79,34)

9,88 (9,75-38,05)

< 0,05

1,39

2,04

4,63

13,18

AURKA

1,0 (0,30-2,58)

40,98 (15,78-91,99)

50,68 (35,51-91,99)

20,49 (15,78-40,13)

< 0,05

0,93

0,77

2,96

6,53

CCNB1

1,0 (0,00-3,14)

37,66 (17,75-72,76)

55,91 (23,02-72,76)

23,26 (17,75-33,24)

< 0,05

1,07

1,51

4,21

13,41

BIRC5

1,0 (0,46-3,40)

70,54 (28,64-179,15)

77,44 (58,69-179,15)

47,50 (28,64-146,52)

NS

1,25

2,23

6,00

14,98

CCNB2

1,0 (0,31-1,93)

41,50 (10,63-108,38)

47,34 (29,55-108,38)

19,97 (10,63-52,53)

NS

0,64

1,28

3,85

10,16

BUB1B

1,0 (0,24-2,97)

36,25 (13,18-94,35)

38,72 (30,91-94,35)

25,99 (13,18-45,10)

NS

0,98

1,37

4,48

17,33

PLK1

1,0 (0,30-2,08)

34,62 (9,99-50,45)

41,93 (22,68-50,45)

14,16 (9,99-37,10)

NS

0,55

0,56

2,62

4,73

TACC3

1,0 (0,00-1,35)

27,86 (9,94-69,07)

37,88 (12,24-69,07)

19,29 (9,94-33,75)

NS

1,82

1,52

3,15

7,50

NDC80

1,0 (0,00-1,39)

24,71 (5,70-209,38)

45,05 (5,70-209,38)

9,62 (6,92-16,37)

NS

1,60

1,10

6,45

17,23

CDC2

1,0 (0,00-2,11)

23,84 (6,26-76,46)

30,34 (8,86-76,46)

23,48 (6,26-13,24)

NS

0,90

0,49

4,13

11,99

NEK2

1,0 (0,16-2,87)

20,70 (5,45-62,25)

20,63 (6,53-62,25)

20,77 (5,45-40,32)

NS

1,71

0,94

4,12

4,37

TTK

1,0 (0,01-7,32)

17,95 (3,23-75,41)

25,75 (6,50-75,41)

12,73 (3,23-21,51)

NS

1,21

4,64

4,68

10,34

ZWINT

1,0 (0,00-3,71)

13,21 (3,97-34,14)

11,69 (3,97-34,14)

13,80 (4,32-17,51)

NS

0,60

0,62

1,64

4,03

CENPE

1,0 (0,03-4,58)

8,46 (0,33-14,83)

10,34 (0,87-12,27)

3,51 (0,33-14,83)

NS

1,09

0,83

1,78

10,08

CCNB3

1,0 (0,00-6,11)

3,87 (0,27-120,26)

3,35 (0,27-120,26)

4,39 (0,50-30,48)

NS

0,33

2,55

0,92

1,56

UBD

1,0 (0,00-3,07)

0,01 (0,00-11,39)

0,01 (0,00-0,13)

0,01 (0,00-11,39)

NS

0,06

2,56

0,26

0,49

MKI67

1,0 (0,03-5,99)

27,75 (7,38-54,07)

29,45 (13,36-54,07)

26,72 (7,38-31,78)

NS

0,95

0,86

3,99

16,00

  1. aMann and Whitney's U Test: ERa-positive cell lines vs ERa-negative cell lines. NS, not significant.
  2. bMedian (range) of gene mRNA levels. The mRNA levels of the tumor cell lines, fibroblasts and epithelial cells samples were normalized such that the median of the 9 normal breast tissues mRNA levels was 1.
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Molecular Cancer

ISSN: 1476-4598