Table 5 Relationship between the mRNA expression levels and DNA amplification levels of the 20 markedly upregulated genes
Genes | Chromosome location | Normal breast tissues (n = 6) | Breast tumors (n = 39) | Unamplified tumors | Amplified tumors | pa | ||
|---|---|---|---|---|---|---|---|---|
Number | mRNA level | Number | mRNA level | |||||
CDC20 | 1p34.1 | 1,0 (0,61-1,28) | 2,89 (0,39-22,65)b | 38 (97,5%) | 2,99 (0,54-22,65) | 1 (2,5%) | 0,39 | NS |
NEK2 | 1q31,2-q41 | 1,0 (0,44-5,23) | 28,41 (2,46-137,03) | 12 (30,8%) | 26,55 (2,66-51,92) | 27 (69,2%) | 28,41 (2,46-137,03) | NS |
BUB1 | 2q14 | 1,0 (0,53-1,51) | 5,52 (0,74-25,63) | 36 (92,3%) | 5,43 (0,74-25,63) | 3 (7,7%) | 6,39 (3,46-17,15) | NS |
TACC3 | 4p16,3 | 1,0 (0,54-2,08) | 7,32 (1,01-29,89) | 38 (97,5%) | 7,09 (1,01-29,89) | 1 (2,5%) | 15,32 | NS |
CENPE | 4q21-q25 | 1,0 (0,02-2,06) | 14,59 (0,08-61,89) | 38 (97,5%) | 14,64 (0,08-61,89) | 1 (2,5%) | 5,54 | NS |
CCNA2 | 4q25-q31 | 1,0 (0,56-1,90) | 10,39 (2,20-37,31) | 36 (92,3%) | 10,12 (2,2-37,31) | 3 (7,7%) | 22,65 (3,81-32,82) | NS |
CCNB1 | 5q12 | 1,0 (0,42-2,32) | 4,18 (0,34-22,47) | 34 (87,2%) | 3,53 (0,34-18,96) | 5 (12,8%) | 8,88 (2,67-22,47) | 0,01 |
UBD | 6p21.3 | 1,0 (0,31-3,91) | 4,75 (0,15-106,40) | 32 (82,1%) | 4,89 (0,15-106,40) | 7 (17,9%) | 2,93 (0,54-7,43) | NS |
TTK | 6q13-q21 | 1,0 (0,71-2,08) | 6,72 (0,61-44,27) | 37 (94,9%) | 6,72 (0,61-44,27) | 2 (5,1%) | 15,91 (5,85-25,96) | NS |
CDC2 | 10q21.1 | 1,0 (0,68-2,66) | 9,52 (1,19-56,17) | 34 (87,2%) | 8,44 (1,19-56,17) | 5 (12,8%) | 17,47 (12,74-42,86) | 0,03 |
ZWINT | 10q21-q22 | 1,0 (0,62-1,97) | 5,38 (1,08-20,70) | 32 (82,1%) | 4,44 (1,08-20,70) | 7 (17,9%) | 12,52 (4,46-18,27) | 0,003 |
BUB1B | 15q15 | 1,0 (0,53-1,69) | 10,48 (1,35-32,33) | 34 (87,2%) | 9,05 (1,35-32,33) | 5 (12,8%) | 17,33 (10,48-27,28) | 0,04 |
CCNB2 | 15q21.2 | 1,0 (0,64-1,95) | 14,21 (2,00-68,51) | 35 (89,7%) | 10,15 (2,0-55,08) | 4 (10,2%) | 21,41 (19,34-68,51) | 0,03 |
PLK | 16p12.1 | 1,0 (0,60-1,87) | 5,46 (0,69-35,59) | 16 (41,0%) | 4,49 (0,69-21,61) | 23 (59,0%) | 5,46 (1,24-35,59) | NS |
AURKB | 17p13.1 | 1,0 (0,90-2,08) | 5,82 (0,00-64,52) | 39 (100%) | 5,82 (0-64,52) | 0 | - | - |
BIRC5 | 17q25 | 1,0 (0,37-2,0) | 14,84 (1,47-150,30) | 27 (69,2%) | 9,96 (1,47-49,07) | 12 (30,8%) | 32,31 (5,84-150,30) | 0,0008 |
NDC80 | 18p11.31 | 1,0 (0,29-1,23) | 6,28 (1,05-126,38) | 34 (87,2%) | 5,69 (1,05-27,22) | 5 (12,8%) | 27,32 (4,13-126,38) | 0,03 |
TPX2 | 20q11.2 | 1,0 (0,59-1,48) | 15,69 (1,65-117,11) | 22 (56,4%) | 10,26 (1,65-34,46) | 17 (43,6%) | 24,03 (5,45-117,11) | 0,002 |
AURKA | 20q13.2-q13.3 | 1,0 (0,36-1,33) | 7,14 (1,46-34,22) | 24 (61,5%) | 5,08 (1,46-32,82) | 15 (38,5%) | 14,04 (3,40-34,22) | 0,002 |
CCNB3 | Xp11 | 1,0 (0,96-4,20) | 5,65 (0,00-61,53) | 33 (84,6%) | 4,76 (0,00-61,53) | 6 (15,4%) | 11,58 (5,16-25,25) | NS |