Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Expression ratio of CCND1 to CDKN2A mRNA predicts RB1 status of cultured cancer cell lines and clinical tumor samples

Figure 3

CCND1/CDKN2A expression predicts RB1 status. (A) RB1 status of 12 cell lines determined by RB1 functional assay. Each cell line was transfected with E2F-regulatory reporter SEAP plasmid with or without the CDKN2A expression vector. The inhibition level of the SEAP reporter gene activity in response to CDKN2A induction was normalized to luciferase activity. (B) RB1 status of 12 cell lines predicted by the expression ratio of CCND1/CDKN2A. mRNAs from the 12 cell lines were analyzed with quantitative RT-PCR analysis for CCND1 and CDKN2A. The threshold of CCND1/CDKN2A to determine RB1 status was established as 0.404 by discriminate analysis. The RB1 statuses determined by CCND1/CDKN2A coincided with those determined by RB1 function assay. (C) Relative RB1 mRNA expression level in 12 cell lines measured with quantitative RT-PCR assay.

Back to article page

Molecular Cancer

ISSN: 1476-4598